ALAS2 Antibody / Erythroid Heme Biosynthesis Enzyme Antibody (RQ6930)

ALAS2 Antibody IF. Immunofluorescent staining of FFPE human PC-3 cells using ALAS2 Antibody / Erythroid Heme Biosynthesis Enzyme Antibody demonstrates granular cytoplasmic green fluorescence with enrichment in a perinuclear distribution, consistent with the mitochondrial localization of ALAS2. ALAS2 is a mitochondrial enzyme that catalyzes the first and rate-limiting step of heme biosynthesis, initiating the pathway required for production of heme and subsequent hemoglobin synthesis. The observed cytoplasmic staining pattern is consistent with localization of ALAS2 within intracellular mitochondrial compartments involved in cellular metabolic activity and heme production. Nuclei are counterstained with DAPI (blue). HIER: steam section in pH 6 citrate buffer for 20 minutes. Green = ALAS2 antibody; Blue = DAPI nuclear stain.

ALAS2 Antibody Multi-Species WB. Western blot analysis of human 293T (lane 1), rat liver (lane 2), rat heart (lane 3), mouse liver (lane 4), and mouse heart (lane 5) lysates using ALAS2 Antibody / Erythroid Heme Biosynthesis Enzyme Antibody demonstrates a prominent immunoreactive band at approximately 65-72 kDa. ALAS2 is a mitochondrial enzyme that catalyzes the first and rate-limiting step of heme biosynthesis, initiating production of 5-aminolevulinic acid required for heme and hemoglobin synthesis. The observed banding pattern across human, rat, and mouse tissues supports cross-species recognition of ALAS2 and is consistent with expression of this highly conserved heme biosynthetic enzyme. The detected molecular weight is in agreement with the expected migration of ALAS2, which is commonly observed at approximately 65-72 kDa depending on species, processing state, and electrophoretic conditions. Predicted molecular weight: approximately 65 kDa. Observed molecular weight: approximately 65-72 kDa.

ALAS2 Antibody FACS. Flow cytometry analysis of human K562 cells stained with ALAS2 Antibody / Erythroid Heme Biosynthesis Enzyme Antibody demonstrates a clear rightward shift of the antibody-stained population (blue) relative to the isotype control (green), consistent with positive detection of ALAS2 expression. ALAS2 is an erythroid-specific mitochondrial enzyme that catalyzes the first and rate-limiting step of heme biosynthesis and is essential for hemoglobin production and erythroid maturation. K562 cells possess erythroid differentiation characteristics and are frequently utilized as a model for studies of hemoglobin synthesis and erythropoiesis. The observed fluorescence shift supports expression of ALAS2 and is consistent with its established role in erythroid lineage biology and heme metabolism. Cells were stained with 1 ug antibody per million cells following blocking with goat serum. Red = cells alone; Green = isotype control; Blue = ALAS2 antibody.