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Home >> Antibodies >> AKT3 Antibody [Discontinued, view alternative antibodies]

AKT3 Antibody [Discontinued, view alternative antibodies] (R31321)

  Catalog No Formulation Size Price (USD)  
Image R31321 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of AKT3 antibody and Lane 1: rat lung; 2: (r) kidney; 3: human HeLa; 4: (h) placenta; 5: (h) A549; 6: mouse NIH3T3 cell lysate. Predicted molecular weight: ~56kDa but can be observed from 60~65kDa.
IHC-P: AKT3 antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of AKT3 antibody and mouse HEPA cells
IHC-P testing of rat kidney tissue. HIER: steamed with pH6 citrate buffer.
IHC-P testing of rat lung tissue. HIER: steamed with pH6 citrate buffer.
Availability Discontinued
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt Q9Y243
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Limitations This AKT3 antibody is available for research use only.
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Description

RAC-gamma serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT3 gene. This gene is mapped to 1q43-q44. The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor(PDGF), insulin, and insulin-like growth factor 1(IGF1). AKT3 plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. This gene is required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the AKT3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 122-136 (TSQIDNIGEEEMDAS) were used as the immunogen for this AKT3 antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the AKT3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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