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Home >> Antibodies >> AKT2 Antibody

AKT2 Antibody (F50042)

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  Catalog No Formulation Size Price (USD)  
Image F50042-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50042-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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AKT2 antibody western blot analysis in 293 lysate. Predicted molecular weight: ~56kDa.
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AKT2 antibody western blot analysis in 293 lysate. Predicted molecular weight: ~56kDa.
Western blot analysis of AKT2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2). Predicted molecular weight: ~56kDa.
Confocal immunofluorescent analysis of AKT2 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
AKT2 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
AKT2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P31751
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
IHC (Paraffin) : 1:10-1:50
Limitations This AKT2 antibody is available for research use only.
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Description

AKT2 is a putative oncogenic protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.

Application Notes

Titration of the AKT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 93-123 from the human protein was used as the immunogen for this AKT2 antibody.

Storage

Aliquot the AKT2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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