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Home >> Antibodies >> AKT1S1 Antibody / PRAS40

AKT1S1 Antibody / PRAS40 (F43688)

  Catalog No Formulation Size Price (USD)  
Image F43688-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F43688-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
AKT1S1 antibody western blot analysis in HeLa lysate. Expected molecular weight ~40 kDa.
AKT1S1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q96B36
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Limitations This AKT1S1 antibody is available for research use only.
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Description

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. [UniProt]

Application Notes

Titration of the AKT1S1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 227-256 from the human protein was used as the immunogen for this AKT1S1 antibody.

Storage

Aliquot the AKT1S1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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