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Home >> Antibodies >> Akt1 Antibody

Akt1 Antibody [clone PKB-175] (R30075)

  Catalog No Formulation Size Price (USD)  
Image R30075 0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS 100 ug 329
Western blot testing of Akt1 antibody and Lane 1: rat ovary; 2: (r) PC-12; 3: human A549; 4: (h) MCF-7; 5: mouse HEPA lysate; Predicted size: 56 kDa; Observed size: 56 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Chicken
Format Ascites
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name PKB-175
Purity Ascites
Gene ID 207
Applications Western blot : 0.25-0.5ug/ml
Limitations This Akt1 antibody is available for research use only.
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Description

PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1(ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. The gene is mapped to chromosome 14q32.3. The protein is critical for ischemic and VEGF-mediated angiogenesis. It regulates pathological angiogenesis, vascular maturation and permeability in vivo.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Akt1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 461-477 of human PKB alpha/Akt1 were used as the immunogen for this Akt1 antibody.

Storage

After reconstitution, the Akt1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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