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Home >> Antibodies >> AKT1 Antibody

AKT1 Antibody (F50039)

  Catalog No Formulation Size Price (USD)  
Image F50039-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F50039-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
AKT1 antibody western blot analysis in T47D lysate
Western blot analysis of AKT1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT1 gene (2).
AKT1 antibody flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P31749
Applications Western blot : 1:1000
Flow Cytometry : 1:10-1:50
Limitations This AKT1 antibody is available for research use only.
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Description

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Application Notes

Titration of the AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 438-468 from the human protein was used as the immunogen for this AKT1 antibody.

Storage

Aliquot the AKT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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