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Home >> Antibodies >> Akt1 Antibody

Akt1 Antibody (F44087)

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  Catalog No Formulation Size Price (USD)  
Image F44087-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F44087-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Akt1 antibody western blot analysis in mouse heart tissue lysate.
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Akt1 antibody western blot analysis in mouse heart tissue lysate.
Akt1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse gallbladder tissue.
Availability 1-3 business days
Species Reactivity Mouse
Predicted Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P31750
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Limitations This Akt1 antibody is available for research use only.
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Description

This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, such as glucose metabolism, glycogen biosynthesis, protein synthesis and turn over, inflammatory response, cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Application Notes

Titration of the Akt1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 22-49 from the mouse protein was used as the immunogen for this Akt1 antibody.

Storage

Aliquot the Akt1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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