- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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AKT1 Antibody detects AKT1, a serine/threonine kinase Akt family member. The family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB-beta or RacPK-beta) and Akt 3 (also designated PKB-gamma or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-beta tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
For a microarray-validated AKT1 antibody supporting high-specificity detection, see our AKT1 antibody (clone AKT1/2552).
Titering of the AKT1 antibody may be required for optimal performance.
A portion of amino acids 85-189 from the human protein was used as the immunogen for the AKT1 antibody.
Store the AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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