AKT1 Antibody [clone AKT1/2491] (V3797)

Western blot testing of human MCF7 cell lysate with AKT1 antibody. Predicted molecular weight ~56 kDa.

SDS-PAGE analysis of purified, BSA-free AKT1 antibody as confirmation of integrity and purity.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using AKT1 antibody. These results demonstrate the foremost specificity of the AKT1/2491 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human pancreas with AKT1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.