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Home >> Antibodies >> AKT Antibody

AKT Antibody (F41829)

  Catalog No Formulation Size Price (USD)  
Image F41829-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F41829-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
AKT antibody western blot analysis in MCF-7 lysate
AKT antibody western blot analysis in mouse cerebellum tissue lysate
AKT antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma
Confocal immunofluorescent analysis of AKT antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
AKT antibody flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Bovine, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P31749
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This AKT antibody is available for research use only.
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Description

In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. [RefSeq].

Application Notes

Titration of the AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 428-457 from human AKT1 was used as the immunogen for this AKT antibody.

Storage

Aliquot the AKT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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