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Home >> Antibodies >> AKAP 95 Antibody / AKAP8

AKAP 95 Antibody / AKAP8 (RQ6599)

  Catalog No Formulation Size Price (USD)  
Image RQ6599 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 389
IHC staining of FFPE human breast cancer tissue with AKAP 95 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendiceal adenocarcinoma tissue with AKAP 95 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with AKAP 95 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric adenocarcinoma tissue with AKAP 95 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gall bladder adenosquamous carcinoma tissue with AKAP 95 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with AKAP 95 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HEK293, 2) PC-3 and 3) HepG2 cell lysate with AKAP 95 antibody. Predicted molecular weight ~76 kDa but routinely observed at ~95 kDa.
Flow cytometry testing of human A431 cells with AKAP 95 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AKAP 95 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O43823
Localization Cytoplasmic, nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This AKAP 95 antibody is available for research use only.
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Description

A-kinase anchor protein 8 is an enzyme that, in humans, is encoded by the AKAP8 gene. This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9.

Application Notes

Optimal dilution of the AKAP 95 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids T380-F546) was used as the immunogen for the AKAP 95 antibody.

Storage

After reconstitution, the AKAP 95 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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