AIF1 Antibody / IBA1 [clone 31A74] (FY12026)

Western blot analysis of AIF1 using anti-AIF1 antibody recombinant rabbit mAb clone 31A74. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIF1 antibody at 1: 500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for AIF1 at approximately 17 kDa. The expected band size for IBA1/AIF1 is at 17 kDa.