- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
AIF1 Antibody for IHC / Allograft inflammatory factor 1 [clone MSVA-955M] (V6070)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V6070-100UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 100 ug | 599.50 | |
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V6070-20UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 20 ug | 324.50 |
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| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG2c, kappa |
| Clone Name | MSVA-955M |
| UniProt | P55008 |
| Localization | Cell projection, Cytoplasm, Cytoskeleton, Phagocytic cup, Ruffle membrane |
| Applications | Immunohistochemistry (FFPE) : 1:100-1:200 |
| Limitations | This AIF1/Allograft inflammatory factor 1 antibody is available for research use only. |
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AIF1 Antibody for IHC clone MSVA-955M recognizes Allograft inflammatory factor 1, also known as IBA1, a cytoplasmic EF-hand calcium-binding protein encoded by the AIF1 gene on chromosome 6p21.3 within the major histocompatibility complex region. Allograft inflammatory factor 1 is widely used as a marker of microglia in the central nervous system and macrophages in peripheral immune tissues. This recombinant antibody is designed to detect endogenous AIF1 protein expression in formalin-fixed, paraffin-embedded tissues.
Allograft inflammatory factor 1, frequently referred to in the literature as IBA1 antibody target protein, belongs to the EF-hand superfamily and contains calcium-binding motifs that regulate actin cytoskeletal remodeling. The protein localizes primarily to the cytoplasm and cellular processes of microglia and macrophages, where it contributes to membrane ruffling, phagocytosis, migration, and inflammatory responses. Expression is low under resting conditions but increases during immune activation, tissue injury, infection, and neurodegeneration.
AIF1 is strongly expressed in resident microglia of the brain and spinal cord, as well as in macrophages within spleen, lymph node, tonsil, and inflamed tissues. During embryonic development, microglial precursors begin expressing AIF1 as they populate the developing nervous system. In pathological contexts such as Alzheimer disease, multiple sclerosis, traumatic brain injury, and tumor-associated inflammation, increased AIF1 expression correlates with activated microglial and macrophage populations.
As an IHC-focused reagent, AIF1 Antibody for IHC clone MSVA-955M supports research requiring visualization of macrophage and microglial distribution in tissue sections. The recombinant format promotes consistency between production lots while targeting AIF1 protein in research applications. Detection of AIF1 can assist investigators studying neuroinflammation, immune cell infiltration, tumor microenvironments, and inflammatory disorders. This antibody targets AIF1 in research applications involving tissue-based analysis of macrophage and microglial populations.
1. Optimal dilution of the AIF1 antibody for IHC should be determined by the researcher.
2. This AIF1/Allograft inflammatory factor 1 antibody is recombinantly produced by expression in CHO cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Purified recombinant human AIF1 protein (around amino acids 1-146) (exact sequence is proprietary) was used as the immunogen for the AIF1/Allograft inflammatory factor 1 antibody.
AIF1/Allograft inflammatory factor 1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.
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