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Home >> Antibodies >> AIF1 Antibody / IBA1

AIF1 Antibody / IBA1 [clone AIF1/2493] (V3809)

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  Catalog No Formulation Size Price (USD)  
Image V3809-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3809-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3809SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human kidney tissue with AIF1 antibody (clone AIF1/2493). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human kidney tissue with AIF1 antibody (clone AIF1/2493). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human spleen tissue with AIF1 antibody (clone AIF1/2493). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free AIF1 antibody (clone AIF1/2493) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2c, kappa
Clone Name AIF1/2493
Purity Protein G affinity chromatography
UniProt P55008
Localization Cytoplasmic, membranous
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This AIF1 antibody is available for research use only.
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Description

AIF1 (Allograft inflammatory factor 1), also called IBA1 (Ionized calcium-binding adapter molecule 1) is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1/IBA1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, it colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding the protein resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).

Application Notes

Titering of the AIF1 antibody may be required for optimal performance.

Immunogen

A portion of amino acids 1-146 from the human protein was used as the immunogen for the AIF1 antibody.

Storage

Store the AIF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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