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Home >> Antibodies >> AIF Antibody / AIFM1

AIF Antibody / AIFM1 [clone 8H1-B10-A12] (F54034)

  Catalog No Formulation Size Price (USD)  
Image F54034-0.1ML In PBS with 50% glycerol and 0.03% ProClin 300 0.1 ml 329
ICC/IF staining of HeLa cells, fixed with 4% Paraformaldehyde, using AIF antibody at 1:200.
Western blot testing of human 1) HeLa, 2) Ramos, 3) HepG2, 4) MCF7 and 5) Jurkat cell lysates using AIF antibody at 1:1000. Predicted molecular weight ~67 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 8H1-B10-A12
Purity Protein G affinity
UniProt O95831
Applications Western blot : 1:1000
ICC/IF : 1:200
Limitations This AIF antibody is available for research use only.
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Description

Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the AIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A human recombinant partial protein was used as the immunogen for this AIF antibody.

Storage

Store the AIF antibody at -20oC.

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