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Home >> Antibodies >> AHR Antibody / Aryl hydrocarbon Receptor

AHR Antibody / Aryl hydrocarbon Receptor (RQ5954)

  Catalog No Formulation Size Price (USD)  
Image RQ5954 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 359
IHC staining of FFPE rat lung with AHR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse lung with AHR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with AHR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with AHR antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) A549, 2) HeLa, 3) PC-3, 4) U-87 MG, 5) Caco-2, 6) rat kidney, 7) mouse heart and 8) mouse NIH 3T3 lysate with AHR antibody. Expected molecular weight ~95 kDa.
Flow cytometry testing of human U-87 MG cells with AHR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AHR antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P35869
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This AHR antibody is available for research use only.
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Description

The aryl hydrocarbon receptor (AhR or AHR or ahr or ahR) is a protein that in humans is encoded by the AHR gene. It is mapped to 7p21.1. The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes.

Application Notes

Optimal dilution of the AHR antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids A6-L842) was used as the immunogen for the AHR antibody.

Storage

After reconstitution, the AHR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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