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Home >> Antibodies >> AHR Antibody

AHR Antibody (F49225)

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  Catalog No Formulation Size Price (USD)  
Image F49225-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
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F49225-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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AHR antibody western blot analysis in (1) mouse NIH3T3 and (2) human T47D cell line lysate. Predicted molecular weight ~ 95kDa.
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AHR antibody western blot analysis in (1) mouse NIH3T3 and (2) human T47D cell line lysate. Predicted molecular weight ~ 95kDa.
AHR antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma.
Confocal immunofluorescent analysis of AHR antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
AHR antibody flow cytometric analysis of NCI-H460 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
  • AHR antibody western blot analysis in (1) mouse NIH3T3 and (2) human T47D cell line lysate. Predicted molecular weight ~ 95kDa.
  • AHR antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma.
  • Confocal immunofluorescent analysis of AHR antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
  • AHR antibody flow cytometric analysis of NCI-H460 cells (green) compared to a <a href=negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis." title="AHR antibody flow cytometric analysis of NCI-H460 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis." onClick="showhideiamges('image4','imagelargedata4',4,4)">
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P35869
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This AHR antibody is available for research use only.
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Description

AHR is a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. [provided by RefSeq].

Application Notes

Titration of the AHR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 555-582 from the human protein was used as the immunogen for this AHR antibody.

Storage

Aliquot the AHR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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