AHR Antibody / Environmental Sensor Protein Antibody (RQ5954)

AHR Antibody Rat Lung IHC. Immunohistochemical staining of FFPE rat lung demonstrates AHR expression using an AHR antibody. AHR, also known as Aryl Hydrocarbon Receptor and an Environmental Sensor Protein, is a ligand activated transcription factor that regulates xenobiotic metabolism, immune responses, and cellular adaptation to environmental stimuli. Distinct cytoplasmic and nuclear staining is observed throughout the bronchial epithelium and alveolar cells, consistent with the role of AHR in pulmonary barrier function and responses to inhaled toxicants. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining. These results support the utility of AHR Antibody for studies of toxicology, inflammation, and environmental sensing pathways.

AHR Antibody Mouse Lung IHC. Immunohistochemical staining of FFPE mouse lung demonstrates AHR expression using an AHR antibody. AHR, also known as Aryl Hydrocarbon Receptor and an Environmental Sensor Protein, is a ligand activated transcription factor that integrates xenobiotic, dietary, and endogenous signals to regulate gene expression and immune homeostasis. Prominent nuclear and cytoplasmic staining is observed in bronchiolar epithelial cells and scattered alveolar cells, consistent with activation of AHR signaling pathways in the pulmonary microenvironment. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining. These findings support the utility of AHR Antibody for studies of toxicology, barrier immunity, and environmental response pathways.

AHR Antibody Human Lung Cancer IHC. Immunohistochemical staining of FFPE human lung cancer demonstrates AHR expression using an AHR antibody. AHR, also known as Aryl Hydrocarbon Receptor and an Environmental Sensor Protein, is a ligand activated transcription factor that regulates xenobiotic metabolism, inflammatory signaling, and cellular responses to endogenous and environmental ligands. Predominantly nuclear and cytoplasmic staining is observed throughout malignant cells, consistent with active AHR signaling and the known involvement of this receptor in tumor progression and metabolic adaptation. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining. These results support the utility of AHR Antibody for studies of toxicology, immune regulation, and lung cancer biology.

AHR Antibody A549 Cells IF. Immunofluorescent staining of FFPE human A549 cells demonstrates AHR expression using an AHR antibody (green). AHR, also known as Aryl Hydrocarbon Receptor and an Environmental Sensor Protein, is a ligand activated transcription factor that mediates cellular responses to xenobiotics, endogenous metabolites, and inflammatory signals. Prominent nuclear and perinuclear fluorescence is observed, consistent with the activation and nuclear translocation of AHR required for transcriptional regulation. DAPI nuclear stain identifies cell nuclei (blue). Heat induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. These results support the utility of AHR Antibody for studies of toxicology, signal transduction, immune regulation, and cancer biology.

AHR Antibody Human Mouse Rat WB. Western blot analysis of AHR expression using an AHR antibody. Lane 1: human A549 cell lysate. Lane 2: human HeLa cell lysate. Lane 3: human PC-3 cell lysate. Lane 4: human U-87 MG cell lysate. Lane 5: human Caco-2 cell lysate. Lane 6: rat kidney tissue lysate. Lane 7: mouse heart tissue lysate. Lane 8: mouse NIH 3T3 cell lysate. AHR, also known as Aryl Hydrocarbon Receptor and an Environmental Sensor Protein, is a ligand activated transcription factor that regulates xenobiotic metabolism and cellular responses to environmental and endogenous signals. A distinct band is detected at approximately 95 kDa in all samples, corresponding to the expected molecular weight of full length AHR. Variations in signal intensity reflect differential expression among tissues and cell types. These results demonstrate conserved AHR expression across human, rat, and mouse samples and support the utility of AHR Antibody for studies of toxicology, signal transduction, and environmental sensing.

AHR Antibody A549 Cells FACS. Flow cytometric analysis of human A549 cells was performed using an AHR antibody following blocking with goat sera. AHR, also known as Aryl Hydrocarbon Receptor and an Environmental Sensor Protein, is a ligand activated transcription factor that regulates xenobiotic metabolism, inflammatory signaling, and cellular responses to endogenous metabolites. Red histogram: cells alone. Green histogram: isotype control. Blue histogram: AHR antibody. The clear rightward shift of the AHR antibody signal relative to both controls demonstrates specific detection of endogenous AHR expression in A549 cells. These results support the utility of AHR Antibody for studies of toxicology, signal transduction, immune regulation, and environmental sensing pathways.