AGO3 Antibody / Argonaute RNA Silencing and RISC Complex Marker [clone PCRP-AGO3-1C5] (V9391)

AGO3 Antibody HeLa IF. Immunofluorescence analysis of PFA-fixed human HeLa cells stained with AGO3 antibody detecting Argonaute 3 (green), clone PCRP-AGO3-1C5. Cytoplasmic puncta consistent with P-bodies and diffuse nucleoplasmic staining are observed, reflecting localization of AGO3 within RNA processing and RISC-associated compartments involved in post-transcriptional gene regulation. Phalloidin counterstain (red) highlights the actin cytoskeleton.

AGO3 Antibody HeLa FACS. Flow cytometry analysis of PFA-fixed human HeLa cells stained with AGO3 antibody detecting Argonaute 3, clone PCRP-AGO3-1C5. The antibody signal (blue) shows a clear rightward shift compared to the isotype control (red), indicating positive intracellular detection of AGO3 following fixation and permeabilization. This pattern is consistent with AGO3 localization as a cytoplasmic and nucleoplasmic component of RNA silencing and RISC-associated complexes.

SDS-PAGE analysis of purified, BSA-free AGO3 antibody (clone PCRP-AGO3-1C5) as confirmation of integrity and purity.

AGO3 Antibody HuProt Microarray. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using AGO3 antibody detecting Argonaute 3, clone PCRP-AGO3-1C5. The antibody shows a strong and highly specific signal for AGO3 with a markedly elevated Z-score and clear separation from lower-ranked proteins, resulting in a high S-score consistent with target specificity. Z-score represents signal intensity in standard deviations above the array mean, while S-score reflects the difference between the top-ranked target and subsequent signals, indicating relative binding specificity of the monoclonal antibody.