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Home >> Antibodies >> AFP Antibody

AFP Antibody (F43722)

  Catalog No Formulation Size Price (USD)  
Image F43722-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F43722-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
IHC analysis of FFPE human hepatocarcinoma tissue stained with AFP antibody
Western blot analysis of AFP antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the AFP gene (2). Predicted molecular weight: ~70kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P02771
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Limitations This AFP antibody is available for research use only.
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Description

Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Application Notes

Titration of the AFP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 300-329 from the human protein was used as the immunogen for this AFP antibody.

Storage

Aliquot the AFP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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