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Home >> Antibodies >> AFP Antibody Cocktail

AFP Antibody Cocktail [clone C2 + C3 + MBS-12] (V2476)

  Catalog No Formulation Size Price (USD)  
Image V2476-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2476-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2476SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
IHC: Formalin-fixed, paraffin-embedded human fetal liver stained with AFP antibody (C2 + C3 + MBS-12).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name C2 + C3 + MBS-12
Purity Protein G affinity chromatography
UniProt P02771
Localization Cytoplasmic
Applications Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This AFP antibody cocktail is available for research use only.
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Description

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This mAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Application Notes

Optimal dilution of the AFP antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human Alpha Fetoprotein was used as the immunogen for the AFP antibody cocktail.

Storage

Store the AFP antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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