AFF4 Antibody / AF4/FMR2 Family Member 4 Antibody (R32620)

AFF4 Antibody Human Placenta IHC. Immunohistochemical staining of FFPE human placental tissue using AFF4 Antibody / AF4/FMR2 Family Member 4 Antibody at 2 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by HRP-conjugated secondary antibody detection and DAB chromogen. Predominantly nuclear staining is observed in placental trophoblast cells, consistent with the expected localization of the transcriptional regulator AFF4. This AFF4 Antibody, also called AF4/FMR2 Family Member 4 Antibody, is suitable for studies of transcription elongation, Super Elongation Complex biology, nuclear protein expression, and gene regulation.

Western blot testing of human 1) HeLa, 2) HepG2, 3) 293T and 4) U-87 MG cell lysate with AFF4 antibody. Predicted molecular weight ~127/98/39 kDa (isoforms 1/2/3).

Flow cytometry testing of fixed and permeabilized human SiHa cells with AFF4 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AFF4 antibody.

Immunoprecipitation of AFF4 protein from 500ug of human 293T whole cell lysate with 2ug of AFF4 antibody.

AFF4 Antibody Mouse Brain IHC. Immunohistochemical staining of FFPE mouse brain tissue using AFF4 Antibody / AF4/FMR2 Family Member 4 Antibody at 2 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by HRP-conjugated secondary antibody detection and DAB chromogen. Predominantly nuclear staining is observed throughout the brain parenchyma, consistent with the expected localization of the transcriptional regulator AFF4. This AFF4 Antibody, also called AF4/FMR2 Family Member 4 Antibody, is suitable for studies of transcription elongation, nuclear protein expression, Super Elongation Complex biology, and gene regulation.

AFF4 Antibody Rat Brain IHC. Immunohistochemical staining of FFPE rat brain tissue using AFF4 Antibody / AF4/FMR2 Family Member 4 Antibody at 2 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by HRP-conjugated secondary antibody detection and DAB chromogen. Predominantly nuclear staining is observed throughout the brain parenchyma, consistent with the expected localization of the transcriptional regulator AFF4. This AFF4 Antibody, also called AF4/FMR2 Family Member 4 Antibody, is suitable for studies of transcription elongation, nuclear protein expression, Super Elongation Complex biology, and gene regulation.

AFF4 Antibody Human, Rat and Mouse WB. Western blot analysis of lane 1: human HeLa, lane 2: human HepG2, lane 3: human 293T, lane 4: human U87, lane 5: rat brain, lane 6: rat C6, lane 7: mouse brain, and lane 8: mouse Neuro-2a lysates using AFF4 Antibody / AF4/FMR2 Family Member 4 Antibody at 0.5 ug/ml. A predominant band is detected at approximately 150 kDa across all samples, although the predicted molecular weight of AFF4 is approximately 128 kDa. The slower migration is consistent with the reported behavior of this large nuclear protein and may reflect post-translational modification or anomalous electrophoretic mobility. This AFF4 Antibody, also called AF4/FMR2 Family Member 4 Antibody, is suitable for studies of transcription elongation, Super Elongation Complex biology, nuclear protein expression, and gene regulation across multiple species.