MLLT5-Associated Factor 1 Antibody / AFF4 Antibody [clone 8G12] (RQ6288)

MLLT5-Associated Factor 1 Antibody Human Cancer Cell WB. Western blot analysis of lane 1: human HeLa, lane 2: human HepG2, lane 3: human Caco-2, lane 4: human HEK293, lane 5: human MDA-MB-453, lane 6: human PANC-1, and lane 7: human SW620 cell lysates using MLLT5-Associated Factor 1 Antibody / AFF4 Antibody. A predominant band is detected at approximately 180 kDa, while the predicted molecular weights for AFF4 isoforms are approximately 127, 98, and 39 kDa. The slower migration is consistent with the known electrophoretic behavior of this large nuclear protein and may reflect post-translational modification or anomalous mobility. This MLLT5-Associated Factor 1 Antibody, also called AFF4 Antibody, is suitable for studies of transcription elongation, Super Elongation Complex biology, and nuclear gene regulation.

MLLT5-Associated Factor 1 Antibody 293T Cell FACS. Flow cytometric analysis of fixed and permeabilized human 293T cells using MLLT5-Associated Factor 1 Antibody / AFF4 Antibody at 1 ug/million cells following blocking with goat sera. The red histogram represents unstained cells, the green histogram the isotype control, and the blue histogram AFF4 antibody staining. A distinct rightward shift of the AFF4-stained population demonstrates specific detection of endogenous AFF4 expression. This MLLT5-Associated Factor 1 Antibody, also called AFF4 Antibody, is suitable for studies of transcription elongation, Super Elongation Complex biology, nuclear protein expression, and flow cytometric analysis of AFF4.