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Home >> Antibodies >> AEG-1 Antibody / Astrocyte elevated gene-1 / MTDH / LYRIC

AEG-1 Antibody / Astrocyte elevated gene-1 / MTDH / LYRIC [clone 2E5G3] (RQ7375)

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  Catalog No Formulation Size Price (USD)  
Image RQ7375 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human colorectal adenocarcinoma tissue with AEG-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with AEG-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) U-87 MG, 2) HeLa, 3) PC-3 and 4) HepG2 cell lysate with AEG-1 antibody. Expected molecular weight: 70-80 kDa.
Flow cytometry testing of human U-87 MG cells with AEG-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AEG-1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 2E5G3
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q86UE4
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This AEG-1 antibody is available for research use only.
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Description

MTDH (Metadherin), also known as protein LYRIC or astrocyte elevated gene-1 protein (AEG-1) is a protein that in humans is encoded by the MTDH gene. AEG-1 is involved in HIF-1alpha mediated angiogenesis. AEG-1 also interacts with SND1 and involved in RNA-induced silencing complex (RISC) and plays very important role in RISC and miRNA functions. AEG-1 induces an oncogene called Late SV40 factor (LSF/TFCP2) which is involved in thymidylate synthase (TS) induction and DNA biosynthesis synthesis. Late SV40 factor (LSF/TFCP2) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP9). AEG-1 acts as an oncogene in melanoma, malignant glioma, breast cancer and hepatocellular carcinoma. It is highly expressed in these cancers and helps in progression and development of these cancers. It is induced by c-Myc oncogene and plays very important role in anchorage independent growth of cancer cells.

Application Notes

Optimal dilution of the AEG-1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids D101-Q270) was used as the immunogen for the AEG-1 antibody.

Storage

After reconstitution, the AEG-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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