AEG-1 Antibody / Astrocyte Elevated Gene-1 Antibody [clone 2E5G3] (RQ7375)

AEG-1 Antibody Human Colorectal Adenocarcinoma IHC. Immunohistochemical staining of FFPE human colorectal adenocarcinoma tissue using AEG-1 antibody demonstrated strong cytoplasmic and membranous staining in malignant epithelial cells, consistent with the elevated expression of astrocyte elevated gene-1 reported in colorectal cancer. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer prior to staining, and the primary antibody was applied following a 20-minute retrieval protocol. This AEG-1 Antibody, also called an Astrocyte Elevated Gene-1 Antibody, is useful for investigating tumor progression, metastasis, oncogenic signaling, chemoresistance, and cancer biology.

AEG-1 Antibody Human Cancer Cell WB. Western blot testing of human U-87 MG (lane 1), HeLa (lane 2), PC-3 (lane 3), and HepG2 (lane 4) cell lysates using AEG-1 antibody detected a prominent band at approximately 70-80 kDa, consistent with the expected migration of astrocyte elevated gene-1. MTDH is commonly observed above its predicted molecular weight due to post-translational modifications, including phosphorylation and glycosylation, and may appear as a closely migrating doublet in some cell types. This AEG-1 Antibody, also called an Astrocyte Elevated Gene-1 Antibody, is useful for investigating tumor progression, metastasis, oncogenic signaling, chemoresistance, and cancer biology.

AEG-1 Antibody Human Glioblastoma Cell FACS. Flow cytometry analysis of fixed and permeabilized human U-87 MG cells using AEG-1 antibody demonstrated a clear rightward shift of the stained cell population (blue) relative to the isotype control (green), indicating specific intracellular detection of astrocyte elevated gene-1. Cells without primary and secondary antibodies served as the blank control (red), and cells were blocked with goat serum prior to staining. This AEG-1 Antibody, also called an Astrocyte Elevated Gene-1 Antibody, is useful for investigating tumor progression, metastasis, oncogenic signaling, chemoresistance, and cancer biology.