ADSL Antibody / Adenylosuccinate lyase Antibody (FY13560)

ADSL Antibody by IHC in human colon carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human colon carcinoma using Adenylosuccinate lyase Antibody reveals strong brown immunoreactivity in tumor epithelial cells, predominantly in the cytoplasmic compartment. Blue hematoxylin counterstaining delineates nuclei and surrounding stromal cells. Heat-mediated antigen retrieval was performed in EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. Immunoreactivity was visualized using an HRP-conjugated secondary antibody and DAB chromogen.

ADSL Antibody by FACS in human 293T cells. Intracellular flow cytometry using Adenylosuccinate lyase Antibody demonstrates a rightward fluorescence shift in fixed and permeabilized 293T cells compared with rabbit IgG isotype control. The antibody-labeled population is shown in blue, isotype control in green, and unstained blank control in red. Cells were fixed with 4% paraformaldehyde, permeabilized, blocked with 10% normal goat serum, and incubated with 1 ug primary antibody per 1 x 10^6 cells for 30 minutes at 20oC. Fluorescent secondary antibody was used for detection.

ADSL Antibody by WB in human and rodent lysates. Western blot analysis using Adenylosuccinate lyase Antibody detects a prominent band near 50 kDa in human Jurkat (lane 1) and 293T (lane 2) cell lysates, rat skeletal muscle (lane 3), and mouse skeletal muscle (lane 4). The observed migration agrees with the approximately 50 kDa calculated molecular weight of ADSL. Reduced lysates containing 30 ug protein per lane were resolved by 10% SDS-PAGE and transferred to nitrocellulose. Following blocking with 5% nonfat milk, membranes were incubated with primary antibody at 0.5 ug/mL overnight at 4oC and developed using HRP-conjugated secondary antibody and chemiluminescence.