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Home >> Antibodies >> ADK Antibody / Adenosine kinase

ADK Antibody / Adenosine kinase (R32499)

  Catalog No Formulation Size Price (USD)  
Image R32499 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of human MCF7 cell lysate with ADK antibody at 0.5ug/ml. Predicted molecular weight ~40 kDa.
IHC testing of FFPE mouse liver with ADK antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat kidney with ADK antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U937 cells with ADK antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ADK antibody.
Flow cytometry testing of human HL60 cells with ADK antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ADK antibody.
Flow cytometry testing of human PC-3 cells with ADK antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ADK antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P55263
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Limitations This ADK antibody is available for research use only.
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Description

Adenosine kinase is an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene.

Application Notes

Differences in protocols and secondary/substrate sensitivity may require the ADK antibody to be titrated for optimal performance.

Immunogen

Amino acids K165-T351 from the human protein were used as the immunogen for the ADK antibody.

Storage

After reconstitution, the ADK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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