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Home >> Antibodies >> ADK Antibody / Adenosine kinase

ADK Antibody / Adenosine kinase (R32499)

  Catalog No Formulation Size Price (USD)  
Image R32499 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 389
Western blot testing of 1) rat liver, 2) rat kidney, 3) rat stomach, 4) rat PC-12, 5) mouse kidney, 6) mouse stomach and 7) mouse NIH 3T3 cell lysate with ADK antibody. Expected molecular weight: 40-45 kDa.
Western blot testing of human 1) placenta, 2) PC-3, 3) T-47D, 4) U937, 5) U-251 MG and 6) HepG2 cell lysate with ADK antibody. Expected molecular weight: 40-45 kDa.
Immunofluorescent staining of FFPE human PC-3 cells with ADK antibody. HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human placenta tissue with ADK antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver tissue with ADK antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver tissue with ADK antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P55263
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Limitations This ADK antibody is available for research use only.
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Description

Adenosine kinase is an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene.

Application Notes

Differences in protocols and secondary/substrate sensitivity may require the ADK antibody to be titrated for optimal performance.

Immunogen

Amino acids K165-T351 from the human protein were used as the immunogen for the ADK antibody.

Storage

After reconstitution, the ADK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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