- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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Adipophilin (PLIN2), also known as adipose differentiation-related protein (ADRP) or Perilipin 2, is a lipid droplet-associated protein that plays a central role in intracellular lipid storage and metabolism. It is widely expressed in cells that accumulate neutral lipids, where it coats lipid droplets and regulates their formation, stability, and utilization. The Adipophilin Antibody / Lipid Droplet Marker is designed to detect PLIN2 in pathways associated with lipid storage, cellular metabolism, and lipid droplet biology across a range of tissue and cell types.
Adipophilin antibody, also referred to as ADRP antibody, PLIN2 antibody, or Perilipin 2 antibody in the literature, enables detection of this protein in both tissue and cell-based assays. Unlike other perilipin family members that are more restricted to adipocytes, PLIN2 is broadly expressed in non-adipose tissues, including macrophages, hepatocytes, and epithelial cells. This widespread expression makes Adipophilin a valuable marker of lipid accumulation in diverse biological systems, including metabolic tissues and immune cell populations.
Functionally, Adipophilin binds to the surface of lipid droplets and regulates access of lipases to stored lipids, thereby controlling lipid turnover and energy homeostasis. Its expression is often upregulated in response to increased lipid availability or metabolic demand, and it plays a role in lipid droplet expansion and stabilization. Adipophilin Antibody is therefore widely used to study lipid metabolism, foam cell formation, and lipid-associated cellular processes in both normal physiology and disease contexts such as atherosclerosis, fatty liver disease, and cancer.
Subcellularly, PLIN2 localizes to the surface of cytoplasmic lipid droplets, where it produces a characteristic punctate or ring-like staining pattern in immunocytochemistry and immunohistochemistry. In flow cytometry applications, intracellular staining following permeabilization allows detection of lipid droplet-associated protein expression in cell populations such as peripheral blood mononuclear cells. In western blot analysis, Adipophilin is detected as a band consistent with its predicted molecular weight, providing a complementary method for assessing expression levels in cell lysates such as Jurkat cells.
This Adipophilin antibody is supported by flow cytometry data demonstrating detection in PBMC populations, western blot data confirming expression in Jurkat cell lysates, and protein microarray specificity validation indicating selective binding to PLIN2 among a large panel of human proteins. These combined validation approaches provide confidence in specificity and performance across multiple assay formats.
Additional Adipophilin antibody reagents targeting ADRP, PLIN2, and Perilipin 2 are available for related research applications.
This antibody is part of a broader antibody panel offered by NSJ Bioreagents.
Optimal dilution of the Adipophilin antibody should be determined by the researcher.
A human recombinant partial protein (amino acids 249-376) was used as the immunogen for this Adipophilin antibody.
Store the Adipophilin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
ADRP antibody, PLIN2 antibody, Perilipin 2 antibody, Adipophilin protein antibody, Lipid droplet protein antibody
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