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Home >> Antibodies >> ADH7 Antibody

ADH7 Antibody (F55038)

  Catalog No Formulation Size Price (USD)  
Image F55038-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 379
Image
F55038-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 155
Western blot testing of human 1) HepG2 and 2) SW480 cell lysate with ADH7 antibody. Predicted molecular weight ~41 kDa.
IHC testing of FFPE human lung tissue with ADH7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human NCI-H460 cells with ADH7 antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human K562 cells with ADH7 antibody; Blue=isotype control, Green= ADH7 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P40394
Localization Cytoplasmic
Applications Western blot : 1:500-1:1000
Immunofluorescence : 1:10-1:50
Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:50-1:100
Limitations This ADH7 antibody is available for research use only.
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Description

This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members.

Application Notes

The stated application concentrations are suggested starting points. Titration of the ADH7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 318-346 from the human protein was used as the immunogen for the ADH7 antibody.

Storage

Aliquot the ADH7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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