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Home >> Antibodies >> ADH5 Antibody / Alcohol dehydrogenase 5

ADH5 Antibody / Alcohol dehydrogenase 5 (F54308)

  Catalog No Formulation Size Price (USD)  
Image F54308-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 360
Image
F54308-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Western blot testing of human 1) K562 and 2) SH-SY5Y cell lysate with ADH5 antibody. Predicted molecular weight ~40 kDa.
IHC testing of FFPE human hepatocarcinoma tissue with ADH5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human breast tissue with ADH5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human placental tissue with ADH5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human K562 cells with ADH5 antibody; Blue=isotype control, Green= antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P11766
Localization Cytoplasmic
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Limitations This ADH5 antibody is available for research use only.
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Description

ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis.

Application Notes

The stated application concentrations are suggested starting points. Titration of the ADH5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 212-239 from the human protein were used as the immunogen for the ADH5 antibody.

Storage

Aliquot the ADH5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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