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Home >> Antibodies >> ADH1C Antibody / Alcohol dehydrogenase 1C

ADH1C Antibody / Alcohol dehydrogenase 1C (F54417)

  Catalog No Formulation Size Price (USD)  
Image F54417-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 359
Image
F54417-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Western blot testing of human T-47D cell lysate with ADH1C antibody. Predicted molecular weight ~40 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with ADH1C antibody.
Immunofluorescent staining of human T-47D cells with ADH1C antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human hepatocarcinoma tissue with ADH1C antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human CCRF-CEM cells with ADH1C antibody; Blue=isotype control, Green= ADH1C antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P00326
Localization Cytoplasmic
Applications Flow cytometry : 1:25 (1x10e6 cells)
Immunofluorescence : 1:25
Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Limitations This ADH1C antibody is available for research use only.
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Description

This gene encodes class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster.

Application Notes

The stated application concentrations are suggested starting points. Titration of the ADH1C antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 231-260 from the human protein was used as the immunogen for the ADH1C antibody.

Storage

Aliquot the ADH1C antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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