- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
APC Antibody ALi 12-28 / APC Tumor Suppressor Antibody [clone ALi 12-28] (V7847)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V7847-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V7847-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
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V7847SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | ALi 12-28 |
| Purity | Protein G affinity chromatography |
| UniProt | P25054 |
| Applications | Flow Cytometry : 1-2ug/10^6 cells in 0.1ml Immunofluorescence : 1-2ug/ml Western Blot : 1-2ug/ml |
| Limitations | This APC Antibody ALi 12-28 / APC Tumor Suppressor Antibody is available for research use only. |
|
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APC Antibody ALi 12-28 / APC Tumor Suppressor Antibody recognizes an N-terminal region of adenomatous polyposis coli (APC), a large tumor suppressor protein with a central role in regulation of canonical Wnt signaling. APC helps control cellular beta-catenin levels and contributes to normal epithelial homeostasis, cell adhesion, migration and cytoskeletal organization. Disruption of APC function is strongly associated with colorectal tumorigenesis and familial adenomatous polyposis.
APC is a core component of the beta-catenin destruction complex, where it works with AXIN, GSK3 and other proteins to promote beta-catenin phosphorylation and degradation. Loss or truncation of APC can disrupt this regulatory process, allowing beta-catenin accumulation and increased transcription of Wnt-responsive genes. These effects help explain why APC alterations are among the most important molecular events in colorectal cancer development.
Clone ALi 12-28 is a well-established mouse monoclonal antibody directed against the N-terminal portion of APC. Epitope-mapping studies localized ALi 12-28 recognition to a region encoded by exons 2-3, making the clone useful for detecting APC forms that retain this N-terminal sequence. Published studies have used ALi 12-28 to investigate endogenous and truncated APC proteins, including experiments in colorectal cancer models.
The N-terminal recognition of this APC Tumor Suppressor Antibody is particularly relevant because many cancer-associated APC mutations generate C-terminally truncated proteins while retaining N-terminal sequences. ALi 12-28 has been used experimentally to detect truncated APC proteins that are not recognized by antibodies directed against lost C-terminal regions.
For a recombinant monoclonal reagent directed against a C-terminal region of APC, see our APC Antibody rEMM43 / Adenomatous Polyposis Coli Antibody page. NSJ Bioreagents offers APC Antibody ALi 12-28 for research into APC expression, tumor suppressor biology and Wnt pathway regulation.
For a recombinant monoclonal reagent directed against a C-terminal region of APC, see our APC Antibody rEMM43 / Adenomatous Polyposis Coli Antibody page.
Optimal dilution of the APC Antibody ALi 12-28 / APC Tumor Suppressor Antibody should be determined by the researcher.
A recombinant human partial protein (amino acids 1-433) was used as the immunogen for the APC Antibody ALi 12-28.
Store the APC Antibody ALi 12-28 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
APC antibody, APC tumor suppressor antibody, Adenomatous polyposis coli antibody, Protein APC antibody
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