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Home >> Antibodies >> ADAR2 Antibody / ADARB1 / RED1

ADAR2 Antibody / ADARB1 / RED1 (RQ7318)

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  Catalog No Formulation Size Price (USD)  
Image RQ7318 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human 293T cells with ADAR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADAR2 antibody.
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Flow cytometry testing of human 293T cells with ADAR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADAR2 antibody.
Flow cytometry testing of human HEL cells with ADAR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADAR2 antibody.
Western blot testing of 1) human SiHa, 2) human HeLa, 3) human HEL, 4) rat brain and 5) mouse brain tissue lysate with ADAR2 antibody. Expected molecular weight: 80-90 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P78563
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ADAR2 antibody is available for research use only.
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Description

Double-stranded RNA-specific editase 1 is an enzyme that in humans is encoded by the ADARB1 gene. This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region.

Application Notes

Optimal dilution of the ADAR2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids K234-R714) was used as the immunogen for the ADAR2 antibody.

Storage

After reconstitution, the ADAR2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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