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Home >> Antibodies >> ADAM9 Antibody

ADAM9 Antibody (RQ6596)

  Catalog No Formulation Size Price (USD)  
Image RQ6596 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 389
Immunofluorescent staining of FFPE human U-2 OS cells with ADAM9 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human bladder adenosquamous carcinoma tissue with ADAM9 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) U-87 MG and 2) A431 cell lysate with ADAM9 antibody. Molecular weight: 90-100 kDa (pro-ADAM9-L form) and 70-80 kDa (ADMA9-L form).
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q13443
Localization Secreted, Cytoplasm (ER)
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This ADAM9 antibody is available for research use only.
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Description

Disintegrin and metalloproteinase domain-containing protein 9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene.

Application Notes

Optimal dilution of the ADAM9 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids R212-D247) was used as the immunogen for the ADAM9 antibody.

Storage

After reconstitution, the ADAM9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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