ACTN4 Antibody / Alpha Actinin 4 (R32459)

Western blot testing of 1) rat skeletal muscle, 2) rat liver, 3) rat lung, 4) rat RH35, 5) mouse skeletal muscle, 6) mouse liver, 7) mouse lung and 8) mouse NIH 3T3 cell lysate with ACTN4 antibody at 0.5ug/ml. Predicted molecular weight ~105 kDa.

Western blot testing of human 1) MCF7, 2) HeLa, 3) HepG2, 4) HT1080, 5) placenta, 6) A549, 7) U-251 MG and 8) RT-4 cell lysate with ACTN4 antibody at 0.5ug/ml. Predicted molecular weight ~105 kDa.

IHC staining of FFPE human colonic adenocarcinoma tissue with ACTN4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate cancer tissue with ACTN4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human HeLa cells with ACTN4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry testing of human U-87 MG cells with ACTN4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACTN4 antibody.