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Home >> Antibodies >> ACTL6A Antibody / BAF53A

ACTL6A Antibody / BAF53A (F52862)

  Catalog No Formulation Size Price (USD)  
Image F52862-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F52862-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Western blot testing of 1) human HeLa, 2) human K562, 3) human Ramos, 4) mouse NIH3T3, 5) rat PC-12 and 6) human testis lysate with ACTL6A antibody at 1:1000. Expected molecular weight: 47-53 kDa.
Fluorescent image of human HeLa cells stained with ACTL6A antibody diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Predicted Reactivity Bovine, Primate
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt O96019
Applications Immunofluorescence : 1:25
Western blot : 1:1000
Limitations This ACTL6A antibody is available for research use only.
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Description

Actin like 6A is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix.

Application Notes

Titration of the ACTL6A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This ACTL6A antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids 306-340 from the human protein.

Storage

Aliquot the ACTL6A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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