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Home >> Antibodies >> Actin Beta Antibody / ACTB

Actin Beta Antibody / ACTB [clone ACTB/1109] (V9727)

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  Catalog No Formulation Size Price (USD)  
Image V9727-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9727-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9727SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human tonsil tissue with Actin Beta antibody (clone ACTB/1109). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with Actin Beta antibody (clone ACTB/1109). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot analysis of human Raji cell lysate using Actin Beta antibody (clone ACTB/1109). Predicted molecular weight ~42 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse and Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name ACTB/1109
Purity Protein A/G affinity
UniProt P60709
Localization Cytoplasm
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Actin Beta antibody is available for research use only.
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Description

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas -Actin and g-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Application Notes

Optimal dilution of the Actin Beta antibody should be determined by the researcher.

Immunogen

Recombinant human protein was used as the immunogen for the Actin Beta antibody.

Storage

Aliquot the Actin Beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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