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Home >> Antibodies >> Actin alpha 1 Antibody / ACTA1

Actin alpha 1 Antibody / ACTA1 [clone ACTA1/360] (V9696)

  Catalog No Formulation Size Price (USD)  
Image V9696-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 499
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V9696-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 219
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V9696SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
IHC staining of FFPE human skeletal muscle with Actin alpha 1 antibody (clone ACTA1/360). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skeletal muscle tissue with Actin alpha 1 (clone ACTA1/360). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human (broad species reactivity predicted)
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name ACTA1/360
Purity Protein A/G affinity
UniProt P68133
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Actin alpha 1 antibody is available for research use only.
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Description

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas b and g are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Application Notes

Optimal dilution of the Actin alpha 1 antibody should be determined by the researcher.

Immunogen

Recombinant human ACTA1 protein was used as the immunogen for the Actin alpha 1 antibody.

Storage

Aliquot the Actin alpha 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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