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Home >> Antibodies >> ACTA2 Antibody Cocktail

ACTA2 Antibody Cocktail [clone 1A4 + ACTA2/791] (V2838)

  Catalog No Formulation Size Price (USD)  
Image V2838-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2838-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2838SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2838IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC: Formalin-fixed, paraffin-embedded rat lung stained with ACTA2 antibody (1A4 + ACTA2/791).
IHC: Formalin-fixed, paraffin-embedded rat stomach stained with ACTA2 antibody.
IHC: Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with ACTA2 antibody.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name 1A4 + ACTA2/791
Purity Protein G affinity chromatography
UniProt P62736
Localization Cytoplasmic
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Limitations This ACTA2 antibody cocktail is available for research use only.
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Description

Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Application Notes

Optimal dilution of the ACTA2 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

An N-terminal decapeptide of the alpha smooth muscle isoform of Actin (1A4) and recombinant full-length human protein (ACTA2/791) were used as the immunogen for the ACTA2 antibody cocktail.

Storage

Store the ACTA2 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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