- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
ACSS2 Antibody / Acetyl-coenzyme A synthetase (RQ7326)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ7326 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q9NR19 |
| Localization | Cytoplasmic, nuclear |
| Applications | Western Blot : 0.5-1ug/ml Flow Cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml |
| Limitations | This ACSS2 antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
ACSS2 antibody recognizes Acetyl-coenzyme A synthetase 2, an essential metabolic enzyme encoded by the ACSS2 gene and responsible for generating acetyl-CoA from acetate. This enzyme localizes to both the cytosol and nucleus, allowing it to participate in lipid synthesis, energy metabolism, and epigenetic regulation through histone acetylation. The human ACSS2 gene resides on chromosome 20q11.22 and is highly expressed in metabolically active tissues including liver, kidney, brain, and adipose tissue. ACSS2-generated acetyl-CoA supports pathways such as fatty acid synthesis, cholesterol synthesis, ketone body utilization, and regulation of chromatin structure through acetylation-dependent gene expression.
Acetyl-coenzyme A synthetase 2 functions by ligating acetate to CoA in an ATP-dependent reaction, creating a direct metabolic link between nutrient availability and intracellular acetyl-CoA pools. Under conditions such as fasting, hypoxia, or increased energy demand, cells rely on ACSS2 to maintain acetyl-CoA homeostasis. In the nucleus, ACSS2 co-localizes with histone acetyltransferases and transcriptional regulators, enabling local production of acetyl-CoA for chromatin modification. This supports transcriptional programs related to stress adaptation, memory formation, neuronal plasticity, and energy-efficient metabolic rewiring. In neurons, ACSS2 is enriched in hippocampus and cortex, where it regulates activity-dependent gene expression and contributes to learning and synaptic maturation.
ACSS2 also plays important roles in cancer biology. Many solid tumors upregulate ACSS2 to utilize extracellular acetate as an alternative carbon source during metabolic stress. This contributes to tumor survival, lipid synthesis, and altered chromatin states associated with growth and metastasis. Elevated ACSS2 expression has been documented in breast cancer, glioblastoma, hepatocellular carcinoma, and renal tumors, where it supports cell proliferation under nutrient-poor conditions. The enzyme further participates in immune and inflammatory pathways by influencing macrophage lipid metabolism, cytokine responses, and oxidative stress adaptation.
At the subcellular level, ACSS2 shows dual localization. In the cytosol, it co-localizes with acetyl-CoA producing complexes and lipid synthesis machinery. In the nucleus, it is frequently observed near chromatin-rich regions and transcription factor complexes that require acetyl-CoA to drive gene activation. Isoform-specific differences have been reported, with variants differing in N-terminal regulatory regions that influence nuclear targeting and response to phosphorylation. Developmentally, ACSS2 expression increases during postnatal growth as metabolic flexibility expands and acetylation-based gene regulation becomes more prominent.
This ACSS2 antibody is suitable for detecting Acetyl-coenzyme A synthetase 2 expression in research addressing metabolic regulation, lipid synthesis, epigenetic control, tumor metabolism, neuronal gene regulation, and stress-adaptive pathways. NSJ Bioreagents provides this reagent as part of its metabolism and cell signaling antibody collection.
Optimal dilution of the ACSS2 antibody should be determined by the researcher.
Recombinant human protein (amino acids E201-A651) was used as the immunogen for the ACSS2 antibody.
After reconstitution, the ACSS2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
