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Home >> Antibodies >> Acrosin Antibody / ACR

Acrosin Antibody / ACR (F51510)

  Catalog No Formulation Size Price (USD)  
Image F51510-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F51510-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Western blot analysis of Acrosin antibody and human Jurkat lysate. Predicted molecular weight ~46 kDa.
IHC analysis of FFPE human testis tissue stained with Acrosin antibody
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P10323
Localization Cytoplasmic
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Limitations This Acrosin antibody is available for research use only.
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Description

Acrosin/ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.

Application Notes

Titration of the Acrosin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 387-416 from the human protein was used as the immunogen for this Acrosin antibody.

Storage

Aliquot the Acrosin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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