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Home >> Antibodies >> ACP3 Antibody / Prostatic acid phosphatase / PAP / ACPP

ACP3 Antibody / Prostatic acid phosphatase / PAP / ACPP (RQ6919)

  Catalog No Formulation Size Price (USD)  
Image RQ6919 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
IHC staining of FFPE human prostate cancer tissue with ACP3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney tissue with ACP3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with ACP3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-87 MG cells with ACP3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human MCF7 cell lysate with ACP3 antibody. Expected molecular weight: 45-50 kDa.
Flow cytometry testing of human HL60 cells with ACP3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACP3 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P15309
Localization Cytoplasmic
Applications Western blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ACP3 antibody is available for research use only.
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Description

Prostatic acid phosphatase (PAP), also prostatic specific acid phosphatase (PSAP), is an enzyme produced by the prostate. This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway.

Application Notes

Optimal dilution of the ACP3 antibody should be determined by the researcher.

Immunogen

Recombinant human PAP/ACP3 protein (amino acids E150-D386) was used as the immunogen for the ACP3 antibody.

Storage

After reconstitution, the ACP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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