ACP3 Antibody for IF / Prostatic Acid Phosphatase Antibody / PAP (RQ6919)

ACP3 Antibody immunohistochemistry analysis of Prostatic acid phosphatase in human prostate cancer tissue. FFPE human prostate carcinoma tissue was stained with ACP3 antibody following heat induced epitope retrieval by boiling sections in pH8 EDTA buffer for 20 minutes prior to cooling and staining. HRP-DAB brown chromogenic signal highlights strong cytoplasmic staining in prostate tumor epithelial cells lining glandular carcinoma structures, consistent with the known expression pattern of Prostatic acid phosphatase (PAP/ACP3) in prostate epithelial differentiation and prostate cancer. Prostatic acid phosphatase immunohistochemistry is widely used in prostate cancer research to identify prostate-derived tumor cells and evaluate PAP expression in prostate carcinoma.

IHC staining of FFPE mouse kidney tissue with ACP3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat kidney tissue with ACP3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

ACP3 Antibody for IF / Prostatic Acid Phosphatase Antibody immunofluorescence analysis of Prostatic acid phosphatase / PAP in human U-87 MG cells. FFPE human U-87 MG cells were stained with ACP3 Antibody for IF (green) following heat induced epitope retrieval by steaming sections in pH6 citrate buffer for 20 minutes. Fluorescent signal reveals cytoplasmic staining consistent with the intracellular localization of Prostatic acid phosphatase (ACP3/PAP). Cell nuclei are counterstained with DAPI (blue), allowing visualization of cellular morphology and nuclear position during immunofluorescence imaging.

Western blot testing of human MCF7 cell lysate with ACP3 antibody. Expected molecular weight: 45-50 kDa.

Flow cytometry testing of human HL60 cells with ACP3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACP3 antibody.