ACO2 Antibody 4C12D1 / Aconitase 2 Monoclonal Antibody [clone 4C12D1] (RQ7281)

ACO2 Antibody 4C12D1 staining of testicular germ cell tumor by IHC. Immunohistochemistry of FFPE human testicular germ cell tumor sections using the ACO2 Antibody. Brown staining is broadly distributed through the tumor cells, with a predominantly granular cytoplasmic appearance consistent with the mitochondrial localization of Aconitase 2. Stromal regions show comparatively less staining, while nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 minutes followed by cooling before testing with this Aconitase 2 Monoclonal Antibody.

ACO2 Antibody 4C12D1 staining of lung adenocarcinoma by IHC. Immunohistochemistry of FFPE human lung adenocarcinoma sections using the ACO2 Antibody. Brown granular cytoplasmic staining is visible throughout the malignant glandular epithelial cells, while nuclei remain predominantly blue. The intracellular distribution is consistent with the mitochondrial localization of Aconitase 2 and clearly delineates the tumor epithelium. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 minutes followed by cooling before testing with this Aconitase 2 Monoclonal Antibody.

ACO2 Antibody 4C12D1 staining of hepatocellular carcinoma by IHC. Immunohistochemistry of FFPE human hepatocellular carcinoma sections using the ACO2 Antibody. Brown granular cytoplasmic staining is visible throughout many of the malignant cells, with variable staining intensity across the tumor and comparatively less signal in the intervening stromal regions. Nuclei are counterstained blue. The predominantly cytoplasmic pattern is consistent with the mitochondrial localization of Aconitase 2 and demonstrates detection with this Aconitase 2 Monoclonal Antibody.

ACO2 Antibody 4C12D1 staining of laryngeal squamous cell carcinoma by IHC. Immunohistochemistry of FFPE human laryngeal squamous cell carcinoma sections using the ACO2 Antibody. Brown cytoplasmic staining is distributed across the malignant epithelial cells, with variable intensity among individual tumor cells and comparatively less signal in intervening stromal areas. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 minutes followed by cooling before testing with this Aconitase 2 Monoclonal Antibody.

ACO2 Antibody 4C12D1 staining of breast cancer by IHC. Immunohistochemistry of FFPE human breast cancer sections using the ACO2 Antibody. Brown cytoplasmic staining is visible throughout the neoplastic epithelial cells, including cells surrounding glandular lumina, with a finely granular appearance consistent with mitochondrial Aconitase 2 localization. Nuclei are counterstained blue and remain readily distinguishable. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 minutes followed by cooling before testing with this Aconitase 2 Monoclonal Antibody.

ACO2 Antibody 4C12D1 staining of mouse cardiac muscle by IHC. Immunohistochemistry of FFPE mouse heart sections using the ACO2 Antibody. Brown cytoplasmic staining is visible throughout the cardiac muscle fibers, consistent with the high mitochondrial content and metabolic demands of cardiomyocytes. Blue nuclear counterstaining provides contrast to the elongated positively stained fibers. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 minutes followed by cooling before testing with this Aconitase 2 Monoclonal Antibody.

ACO2 Antibody 4C12D1 staining of rat cardiac muscle by IHC. Immunohistochemistry of FFPE rat heart sections using the ACO2 Antibody. Strong brown cytoplasmic staining extends along the cardiac muscle fibers, with a distribution consistent with abundant mitochondrial Aconitase 2 in metabolically active cardiomyocytes. Nuclei are counterstained blue and remain distinct from the predominantly cytoplasmic signal. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 minutes followed by cooling before testing with this Aconitase 2 Monoclonal Antibody.

ACO2 Antibody 4C12D1 detection of Aconitase 2 across human, rat and mouse samples by WB. Western blot testing with the ACO2 Antibody. Lane 1: human HeLa; lane 2: human U-87 MG; lane 3: human Jurkat; lane 4: human SH-SY5Y; lane 5: rat skeletal muscle; lane 6: rat brain; lane 7: mouse skeletal muscle; lane 8: mouse brain. A strong predominant band is detected at approximately 85 kDa across all eight samples, closely matching the predicted molecular weight of ACO2. The clean cross-species signal demonstrates recognition of mitochondrial Aconitase 2 by clone 4C12D1 in human, rat and mouse lysates.