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Home >> Antibodies >> ACK1 Antibody

ACK1 Antibody (F54942)

  Catalog No Formulation Size Price (USD)  
Image F54942-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 359
Image
F54942-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q07912
Applications Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Limitations This ACK1 antibody is available for research use only.
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Description

ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.

Application Notes

The stated application concentrations are suggested starting points. Titration of the ACK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 10-39 from the human protein was used as the immunogen for the ACK1 antibody.

Storage

Aliquot the ACK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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