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Home >> Antibodies >> Acidic Cytokeratin Antibody

Acidic Cytokeratin Antibody [clone SPM115] (V7398)

  Catalog No Formulation Size Price (USD)  
Image V7398-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V7398-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V7398SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V7398IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC staining of FFPE human tonsil tissue with Acidic Cytokeratin antibody (clone SPM115). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM115
Purity Protein G purified
UniProt Q7Z794
Localization Cytoplasmic
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This Acidic Cytokeratin antibody is available for research use only.
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Description

This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. MAb SPM116 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Acidic Cytokeratin antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Solubilized keratin extract from human stratum corneum was used as the immunogen for the Acidic Cytokeratin antibody.

Storage

Store the Acidic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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