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Home >> Antibodies >> Acidic Cytokeratin Antibody / LMW / Type I

Acidic Cytokeratin Antibody / LMW / Type I [clone rKRTL/8751] (V4484)

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  Catalog No Formulation Size Price (USD)  
Image V4484-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4484-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4484SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human placental tissue with Acidic Cytokeratin antibody (clone rKRTL/8751). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human placental tissue with Acidic Cytokeratin antibody (clone rKRTL/8751). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Acidic Cytokeratin antibody (clone rKRTL/8751) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rKRTL/8751
Purity Protein A/G affinity
UniProt Q7Z794
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This Acidic Cytokeratin antibody is available for research use only.
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Description

This mAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 5048, 46, 45, and 40kDa.

Application Notes

Optimal dilution of the Acidic Cytokeratin antibody should be determined by the researcher.

Immunogen

Human epidermal keratin was used as the immunogen for the Acidic Cytokeratin antibody.

Storage

Aliquot the Acidic Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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