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Home >> Antibodies >> Acidic Cytokeratin Antibody [Discontinued, view alternative antibodies]

Acidic Cytokeratin Antibody [Discontinued, view alternative antibodies] [clone KRTL/1377] (V3533)

  Catalog No Formulation Size Price (USD)  
Image V3533-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V3533-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V3533SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V3533IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of FFPE human skin with acidic Cytokeratin antibody (clone KRTL/1377). Staining of FFPE tissue requires boiling sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE Analysis of Purified, BSA-Free Acidic Cytokeratin Antibody (clone KRTL/1377). Confirmation of Integrity and Purity of the Antibody.
Availability Discontinued
Species Reactivity Human. Others not tested but broad species reactivity expected.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name KRTL/1377
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 374454
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Flow Cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This acidic Cytokeratin antibody is available for research use only.
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Description

This mAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the acidic Cytokeratin antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A human partial recombinant KRT77 protein was used as the immunogen for this acidic Cytokeratin antibody.

Storage

Store the acidic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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