Acidic Cytokeratin Antibody [clone AE1] (V2328)

Description

Acidic Cytokeratin antibody clone AE1 is a monoclonal antibody that detects a range of low molecular weight cytokeratins belonging to the acidic type I family, including CK10, CK14, CK15, CK16, CK18, and CK19. These keratins are expressed in simple epithelia and certain stratified epithelial tissues, where they play roles in providing structural integrity and regulating cell differentiation. Because of their broad expression patterns, acidic cytokeratins serve as valuable markers for studying epithelial biology and epithelial-derived tumors. NSJ Bioreagents provides Acidic Cytokeratin antibody clone AE1 for applications in cancer pathology, developmental research, and epithelial cell studies.

Acidic Cytokeratin antibody clone AE1 produces strong cytoplasmic staining in epithelial tissues, reflecting the distribution of type I keratins. Its wide reactivity across multiple acidic keratins makes it an essential reagent in pathology. In diagnostic settings, clone AE1 is frequently used to characterize carcinomas, particularly adenocarcinomas, and to distinguish epithelial tumors from those of mesenchymal or hematopoietic origin.

In cancer research, Acidic Cytokeratin antibody clone AE1 is used to study keratin expression patterns in breast, lung, gastrointestinal, and ovarian tumors. Shifts in acidic cytokeratin expression often correlate with tumor progression and epithelial to mesenchymal transition, processes linked to metastasis. Clone AE1 helps researchers investigate how changes in cytokeratin composition influence cancer behavior.

Beyond oncology, Acidic Cytokeratin antibody clone AE1 has applications in developmental biology and regenerative medicine. Acidic keratins are expressed at specific stages of epithelial differentiation, and detection with this antibody provides insights into how epithelial tissues mature and repair themselves following injury.

This antibody has also been employed in studies of epithelial stress responses, as acidic keratins participate in protecting cells against physical and chemical stress. Their expression can be altered by tissue injury, infection, or chronic disease, and clone AE1 has been used to monitor these changes.

Validated for both tissue-based and cell-based studies, Acidic Cytokeratin antibody clone AE1 delivers reproducible cytoplasmic staining patterns. It is widely cited in cancer pathology, developmental research, and epithelial biology. Alternate names include type I cytokeratin antibody, low molecular weight cytokeratin antibody, and keratin AE1 antibody.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Acidic Cytokeratin antibody AE1 to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Solubilized keratin extract from human stratum corneum

Storage

Store the Acidic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).