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Home >> Antibodies >> Acid Sphingomyelinase Antibody / SMPD1

Acid Sphingomyelinase Antibody / SMPD1 (F54362)

  Catalog No Formulation Size Price (USD)  
Image F54362-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 369
Image
F54362-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Western blot testing of human 1) K562, 2) MCF7, 3) HepG2 and 4) A431 cell lysate with Acid Sphingomyelinase antibody. Predicted molecular weight ~70 kDa.
Western blot testing of human 1) cerebellum, 2) K562, 3) HepG2 and 4) MCF7 cell lysate with Acid Sphingomyelinase antibody. Predicted molecular weight ~70 kDa.
IHC testing of FFPE human breast tissue with Acid Sphingomyelinase antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human K562 cells with Acid Sphingomyelinase antibody; Blue=isotype control, Green= Acid Sphingomyelinase antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
UniProt P17405
Applications Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Flow cytometry : 1:25 (1x10e6 cells)
Limitations This Acid Sphingomyelinase antibody is available for research use only.
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Description

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq].

Application Notes

The stated application concentrations are suggested starting points. Titration of the Acid Sphingomyelinase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 391-419 from the human protein was used as the immunogen for the Acid Sphingomyelinase antibody.

Storage

Aliquot the Acid Sphingomyelinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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